QIAGEN QIAamplifier 96

Description

QIAGEN QIAamplifier 96 is a fast and high-performance 96-well block system for all end-point PCR requirements. It is equipped with a heated smart lid for consistency of results. Low noise with a maximum of 45 decibels when running makes it a low-profile lab companion. The QIAamplifier 96 is designed to last and will deliver consistent and reproducible results run after run.
For fast and high-performance end-point PCR experiments in a 96-well format
  • Fast cycling protocol, down to 45 minutes for 30 cycles
  • Temperature ramping rates up to 4°C/s
  • Temperature uniformity down to ±0.2°C across the sample block
  • Linear gradient function for assay optimization

QIAamplifier 96 Performance

The QIAamplifier 96 is a high-performance thermal cycler with a 96-well block and a gradient function ideal for all your end-point PCR requirements. High expectations for long-term reliability and robustness are met with the well-thought-out design and assembly.

Principle

The QIAamplifier 96 is a stand-alone end-point PCR cycler with a 7-inch color touchscreen and an easy-to-use software interface. Users can choose between different programming modes and templates and a quick-start list.

Fast ramping, highest accuracy block control

The QIAamplifier 96 controls the sample block temperature without under- or overshooting the programmed target temperature. The innovative temperature control system maximizes experimental reproducibility and achieves outstanding accuracy.

  • High cooling and heating rates (3.3°C/s and 4°C/s respectively) for short protocol run times
  • Control accuracy: ±0.1°C
  • Temperature uniformity down to ±0.2°C at 55°C after 15 s
  • Reaction mix reaches set target temperature with the best match of a set and real data

High-performance smart lid

The heated lid of the QIAamplifier 96 thermal cycler is equipped with smart lid technology with a temperature range from 30°C to 110°C. An integrated slip clutch always maintains constant contact pressure, regardless of the shape and height of the plasticware. This optimizes the contact between the sample block well and the walls of the plasticware, resulting in reproducible conditions. A rubber seal in the lid encapsulates the space surrounding the sample block. This closed space increases the sample block temperature uniformity and prevents condensation formation during the final PCR cooling step. A short equilibration period during the first heating of the lid also contributes to a completely balanced atmosphere above the sample block.

Linear Gradient Tool for assay optimization

The Linear Gradient Tool facilitates finding the optimum annealing temperature under experimental conditions. The desired temperature increment for the gradient is programmed after the entry of an annealing temperature for the block center. The specific block design and electronics execute a temperature gradient along the long side of the block as linear as technically possible. This means the maximum use of the gradient results.

  • Gradient temperature range: 20°C to 99°C
  • Maximum gradient 20°C across the block with 0.1°C increments
  • Control accuracy: ±0.1°C

Robust and designed to last

The sample block of the QIAamplifier 96 is perfectly sealed to prevent condensation from coming into contact with either the Peltier elements located below the sample block or with other electronic components. This grants protection from corrosion and condensation and extends the lifetime of the instrument.

Whisper quiet

The airflow of the QIAGEN QIAamplifier 96 is optimized to keep the maximum noise level of the instrument down to an extremely low 45 decibels. This efficient airflow system also means that the cycler takes up very little space. In addition to the compact footprint, a small clearance zone of just 10 cm is required for sufficient airflow – much lower than that of other thermal cyclers.

Procedure

The QIAamplifier 96 enables fast end-point PCR in tubes, strips, and 96-well plate format. The QIAamplifier 96 is designed for convenience, ease of use, and reliability and seamlessly integrates any new or existing PCR workflow.

The flexibility of chemistries and plasticware

The QIAamplifier 96 is an open system and can be used in combination with any compatible PCR kit. The SBS sample block allows the QIAamplifier 96 to accommodate most tubes, strips, and low-/normal-profile plates. Discover the joy of hassle- and optimization-free PCR with the QIAamplifier 96 and AllTaq Master Mix and PCR Core Kits. Harness the power of multiplexing with UCP Multiplex PCR kits and the QIAamplfier 96. Try the QIAGEN One-Step Ahead RT-PCR Kit with the QIAamplifier 96 for faster one-step RT-PCR with high sensitivity, specificity, and fidelity.

Intuitive programming

The QIAGEN QIAamplifier 96 Software is optimized for fast setup of PCR protocols. The Software includes several features for easy programming and intuitive handling:

  • Pre-installed program templates can be directly used for an application or can be adapted easily
  • Multi-step programming allows users to quickly edit all parameters for every program step within a single screen
  • Direct spreadsheet and graphical programming parameters are shown on a single screen; one-touch leads from the spreadsheet to the graphical programming mode

Applications

The QIAGEN QIAamplifier 96 is suitable for any end-point PCR application in molecular biology workflows such as genotyping, target detection, semi-quantitative gene expression, reverse transcription, and multiplex PCR.

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