Description
QIAcube HT enables automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology. Users can quickly and easily purify DNA, RNA, and miRNA from almost any type of sample — including cells,tissues, and food material, as well as from bacteria and viruses in animal samples. Automated instrument protocols and dedicated QIAcube HT purification kits and plasticware increase reliability and convenience and save valuable time. The system provides the same high-quality results as other trusted QIAGEN purification technologies.
Performance
QIAcube HT provides fast and reliable automated high-throughput nucleic acid purification for use in a variety of sensitive downstream applications. Researchers can easily increase their sample purification throughput and expect the same high-quality results as from other trusted QIAGEN nucleic acid purification solutions.
Procedure
QIAcube HT provides automated, high-throughput nucleic acid purification for 24–96 samples per run, in increments of 8 samples. The instrument uses a vacuum-driven system to perform the simple and fast bind–wash–elute procedure during sample purification. The vacuum chamber is composed of two separate compartments. This configuration is one of many measures that minimizes sample cross-contamination during purification.
All materials needed for sample purification are placed onto the instrument worktable prior to the run. A 96-well sample block contains the starting sample material that will be lysed and transferred to the filter plate during the run. Easy-to-fill disposable troughs contain all necessary buffers and reagents for sample binding, washing, and elution. Pipet tips are positioned on the right side of the worktable.
| Features | Specifications |
| Accessories (supplied) | Vacuum Control Station, Vacuum Chamber, Pinch Valve, Channeling Adapter (Channeling Block and Channeling Block Holder), Transfer Carriage, Riser Block EMTR, Adapter Reagent Plate SBS, Adapter Reagent Plate R1, Tip Disposal Box Holder, Tip Chute, Tip Chute Protective Cap, Waste Bottle with Lid Assembly |
| Communications | RS232 and USB |
| Consumables (supplied) | QIAcube HT Plasticware (5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad), 3x Tip Disposal Box with Lid, 2x Reagent Trough Lid 170 ml, 3x Reagent Trough with Lid 70 ml |
| Dimensions | Instrument width: 540 mm (21 in.), height: 450 mm (18 in.), depth: 630 mm (25 in.); vacuum control station width: 495 mm (19 in.), height: 520 mm (20 in.), depth: 400 mm (16 in.) |
| Electrical | 100–240 V AC, 250 VA, 50–60 Hz, overvoltage category II; mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages |
| HEPA filter | Preinstalled dual-cartridge 3M Air-Mate HEPA filter system provides positive pressure clean air within the closed hood. |
| Humidity | Operating humidity 40-70% |
| Notebook (supplied) | QIAcube HT comes with a notebook computer for added flexibility. |
| Pipetting system | 8 pipetting channels, pipetting volume (20-200 µl) |
| Place of operation | For indoor use only |
| Pollution level | 2 |
| Precision | 20-200 µl, less than 4% coefficient of variation |
| Software | Supplied with unlimited user license with free upgrades by web download |
| Table capacity | 4 x SBS positions (127.76 x 85.48 mm), compatible with 96-well plates or tip racks 1 custom adapter for a reagent trough plate compatible to accept reagent troughs vacuum chamber with chambers for waste and elution |
| Throughput | 24-96 samples in increments of 8 samples |
| Tip disposal | All used tips ejected externally; no waste accumulation on the worktable |
| UV lamp | Preinstalled UV lamp for worktable decontamination |
| Warranty | 1-3 years warranty, depending on service package |
| Weight | QIAcube HT instrument: 41 kg (90 lb.); vacuum control station: 19 kg (42 lb.) |
