LONZA Nucleofector ™

Description

Nucleofection ™ is a technology based on the momentary creation of small pores in cell membranes by applying an electrical pulse. The comprehensive way in which Nucleofector™ Programs and cell-type-specific solutions are developed enables nucleic acid substrate delivery not only to the cytoplasm but also through the nuclear membrane and into the nucleus. This allows for high transfection efficiencies up to 99% and makes the transfection success independent from cell proliferation.

4D-Nucleofector™ Core Unit

The 4D-Nucleofector Core Unit is the controlling unit of the 4D-Nucleofector System. The different functional units can be added in a modular way to the 4D-Nucleofector Core Unit and each is suited for different applications:

  • X Unit: Supporting Nucleofection of various cell numbers in different formats (16-well Nucleocuvette Strips or 100 µL Nucleocuvette
  • Y Unit: Enabling adherent Nucleofection in 24-well culture plates.
  • LV Unit: Large-scale transfection of up to 1×109 cells
  • 96-well ShuttleTMAdd-on: Higher throughput Nucleofection in 96-well Nucleocuvette Plates

Transfections per minute:

  • X Unit (16-well NucleocuvetteStrips): 16-32 reactions
  • X Unit (100 µL NucleocuvetteVessels): 20-26 reactions
  • Y Unit (Dipping Electrodes):8-10 reactions
  • LV Unit (LV NucleocuvetteCartridges): 2-3 mL

X Unit

The 4D-Nucleofector X Unit is one of the three functional modules of the 4D-Nucleofector System. It supports Nucleofection of various cell numbers in different formats. The 4D-Nucleofector X Unit is also required in order to run the 96-well Shuttle Device.

Y Unit

Electroporation-based methods have so far required cells to be in suspension for transfection. The Lonza Nucleofector Technology entered a new era and allows direct Nucleofection of cells in adherence. Cells that typically grow in adherence to cell culture can be kept and transfected by Nucleofection in their physiological state. The Y Unit of the 4D-Nucleofector System works with disposable conductive polymer Dipping Electrode Arrays that can be inserted into standard 24-well culture plates for Nucleofection.

LV Unit

Large-Scale Transfection Using the 4D-Nucleofector LV Unit – Experience the new functional unit for the 4D-Nucleofector System which expands our proven system to larger-scale transfection. The LV Unit allows for closed, scalable transfection of larger cell numbers in the range of 1×107 to 1×109 cells. Transfection protocols can be established on a smaller scale using the X Unit and subsequently transferred to the LV Unit without the need for re-optimization. Transferability has been tested for various cell types, including human T cells, CHO-S, HEK293-S, or K-562.

96-well Shuttle System

The 96-well Shuttle System is a medium-throughput add-on for the 4D-Nucleofector System suited for convenient optimization of Nucleofection conditions or as an assay establishment tool.

The complete system consists of three components:

  • The 4D-NucleofectorSystem (Core Unit, cat. no. AAF-1002B, and X Unit, cat. no. AAF-1002X) serving as the program delivery unit
  • The 96-well ShuttleSystem which mediates the transfer of the respective 96-well program to a specific well of the 96-well Nucleocuvette Plate
  • A laptop computer with the 96-well Shuttle
  • The software controlling the interaction between the devices

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