Dot and Slot Blottersadmin
Fast sample-to-membrane hybridisation guaranteed for immediate screening
Available in alphanumeric 48 and 96 sample throughputs, Scie-Plas’s dot and slot blot microfiltration manifolds provide an easy and reproducible means to hybridise proteins and nucleic acids in solution onto membranes. These manifolds, which are machined from high-density acrylic, have precision-lapped mating surfaces that ensure uniform filter/membrane contact without gaskets and ‘O’ rings. Manifold assembly is simple, being achieved by the alignment and tightening of stainless steel thumbscrews and mating screws, while filters can be cut down to the exact size of the template. Standard manifold configurations are available with the DHM-48 and DHM-96 units providing 48 and 96 dots, 3mm in diameter, that are compatible with the configuration of 96-well microtitre plates. The SHM-48 unit focus up to 48 samples into thin lines, less than 0.5 x 6mm in size, making densitometric quantitation more reproducible. Each hybridisation manifold requires a vacuum pump or water aspirator equivalent to 600mm Hg (0.8 Bar).