Densitometer

DEN-1, Densitometer (suspension turbidity detector)

1

Description

Densitometers are designed for measurement of cell suspension’s turbidity in the range:

DEN-1: 0.3–5.0 McFarland units
(100 × 106–150 × 107 cells/ml);

Densitometers provide the opportunity to measure solution turbidity in a wider range (up to 15.0 McFarland units) however, it is necessary to remember that in this case the standard deviation values increase. A densitometer is used for measurement of cell concentration (bacterial, yeast cells) during fermentation process, determination of microorganism sensitivity to antibiotics, microorganism identification using various test-systems, for measurement of absorption at the definite wavelength, as well as for quantitative estimation of concentration of colour solution, absorbing green light. The operation principle is based on measurement of optical density with digital presentation of  results in McFarland units. The unit is calibrated at the factory (for operation with 18 mm diameter glass tubes) and keeps calibration without power supply. However, if necessary it is possible to calibrate the unit by 2–6 points in 0.5–5.0 McFarland unit range. Both commercial standards (e.g. produced by BioMerieux, Lachema, etc.) and the cell suspensions prepared in a laboratory can be used for calibration.

Following calibration kits are available on request:

  • CKG16 for glass tubes with diameter 16 mm;
  • CKG18 for glass tubes with diameter 18 mm.

Two versions of the product are available:

  1. DEN-1 powered from external energy supply;
  2. DEN-1B powered both from external energy supply and from batteries (АА). Besides, DEN-1B operates with higher precision of measurements (up to 0,01 McF).

Specification

Light source

LED

Wavelength (λ)

λ = 565 ±15 nm

Measurement range

0.3–15.0 McF

Display resolution

0.1 McF

Accuracy, of the full scale

±3%

Measurement time

1 sec

Sample volume

not less than 2 ml

Tube external diameter

18 mm (without adapter) or 16 mm (using optional A-16 adapter)

Display

LED

Overall dimensions

165 × 115 × 75 mm

Weight

0.7 kg

Input current/power consumption

12 V, 80 mA / 1 W

External power supply

Input AC 100–240 V, 50/60 Hz; Output DC 12 V

Standard set

External power supply

DEN-1B, Densitometer (suspension turbidity detector)

23

Description

Densitometers are designed for measurement of cell suspension’s turbidity in the range:

DEN-1B: 0.0–6.0 McFarland units
(0–180 × 107 cells/ml);

Densitometers provide the opportunity to measure solution turbidity in a wider range (up to 15.0 McFarland units) however, it is necessary to remember that in this case the standard deviation values increase. A densitometer is used for measurement of cell concentration (bacterial, yeast cells) during fermentation process, determination of microorganism sensitivity to antibiotics, microorganism identification using various test-systems, for measurement of absorption at the definite wavelength, as well as for quantitative estimation of concentration of colour solution, absorbing green light. The operation principle is based on measurement of optical density with digital presentation of  results in McFarland units. The unit is calibrated at the factory (for operation with 18 mm diameter glass tubes) and keeps calibration without power supply. However, if necessary it is possible to calibrate the unit by 2–8 points in 0–6.0 McFarland unit range. Both commercial standards (e.g. produced by BioMerieux, Lachema, etc.) and the cell suspensions prepared in a laboratory can be used for calibration.

Following calibration kits are available on request:

  • CKG16 for glass tubes with diameter 16 mm;
  • CKG18 for glass tubes with diameter 18 mm.

Two versions of the product are available:

  1. DEN-1 powered from external energy supply;
  2. DEN-1B powered both from external energy supply and from batteries (АА). Besides, DEN-1Boperates with higher precision of measurements (up to 0,01 McF).

Specification

Light source

LED

Wavelength (λ)

λ = 565 ±15 nm

Measurement range

0.00–15.00 McF

Display resolution

0.01 McF

Accuracy, of the full scale

±3%

Measurement time

1 sec

Sample volume

not less than 2 ml

Tube external diameter

18 mm (without adapter) or 16 mm (using optional A-16 adapter)

Display

LCD

Overall dimensions

165 × 115 × 75 mm

Weight

0.7 kg

Independent power supply

3 × AA batteries

Input current/power consumption

12 V, 7 mA / 0.1 W

External power supply

Input AC 100–240 V, 50/60 Hz; Output DC 12 V

Standard set

External power supply and 3 × AA batteries

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